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C9745

Sigma-Aldrich

Anti-Cullin 3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-CUL3

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About This Item

Código UNSPSC:
12352203
conjugado:
unconjugated
application:
IF
IP
WB
clone:
polyclonal
reatividade de espécies:
rat, human, mouse
citations:
1
técnica(s):
immunoprecipitation (IP): 1-2 μg using lysate of rat brain
indirect immunofluorescence: 2-5 μg/mL using mouse 3T3 cells
western blot: 2-5 μg/mL using whole extract of human Jurkat cells

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

peso molecular

antigen ~80 kDa

reatividade de espécies

rat, human, mouse

concentração

~1.0 mg/mL

técnica(s)

immunoprecipitation (IP): 1-2 μg using lysate of rat brain
indirect immunofluorescence: 2-5 μg/mL using mouse 3T3 cells
western blot: 2-5 μg/mL using whole extract of human Jurkat cells

nº de adesão UniProt

Q13618

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CUL3(8452)
mouse ... Cul3(26554)
rat ... Cul3(301555)

Especificidade

Anti-Cullin 3 (C-terminal) recognizes human, mouse, and rat Cullin 3.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análise (COA)

Lot/Batch Number
047M4846V
029K4759

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Lionel Pintard et al.
Nature, 425(6955), 311-316 (2003-09-19)
Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box)
Lionel Pintard et al.
The EMBO journal, 23(8), 1681-1687 (2004-04-09)
Cullin-based E3 ligases target substrates for ubiquitin-dependent degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box) and ECS (ElonginC-Cul2-SOCS box) complexes are so far the best-characterized cullin-based ligases. Their atomic structure has been solved recently, and several substrates have been described
Wananit Wimuttisuk et al.
Molecular biology of the cell, 18(3), 899-909 (2006-12-29)
Cullins are members of a family of scaffold proteins that assemble multisubunit ubiquitin ligase complexes to confer substrate specificity for the ubiquitination pathway. Cullin3 (Cul3) forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of
J D Singer et al.
Genes & development, 13(18), 2375-2387 (1999-09-29)
Cyclin E is an unstable protein that is degraded in a ubiquitin- and proteasome- dependent pathway. Two factors stimulate cyclin E ubiquitination in vivo: when it is free of its CDK partner, and when it is phosphorylated on threonine 380.
M T M van Jaarsveld et al.
Oncogene, 32(36), 4284-4293 (2012-10-10)
Epithelial ovarian cancer is the most lethal gynecological malignancy in the Western world. A major impediment for the successful treatment is the development of drug resistance. The molecular processes that contribute to resistance have been extensively studied; however, there is
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