B8434

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

• Sinônimo(s): Monoclonal Anti-BrdU antibody produced in mouse, Anti Brdu, Brdu Antibody, Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal, Anti-Bromodeoxyuridine
• Número MDL: MFCD00164550
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture; purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: BU-33, monoclonal
• forma: buffered aqueous solution
• reatividade de espécies: wide range
• embalagem: antibody small pack of 25 μL
• concentração: ~1.5 mg/mL
• técnica(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU
• Isotipo: IgG1
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Especificidade

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Imunogênio

bromodeoxyuridine conjugated to KLH.

Aplicação

Anti-BrdU antibody has been used in:

• BrdU labeling
• immunohistochemistry
• immunostaining
• BrdU assay

Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:

• to detect 5-fluorouridine-labelled RNA
• to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
• against BrdU labelled rat retinal sections

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable