10280879001

5-Bromo-2′-desoxiuridina

>97%, crystalline solid, pkg of 1 g

• Sinônimo(s): 5-BrdU, 5-Bromo-1-(2-desoxi-β-D-ribofuranosil)uracila, 5-Bromouracila desoxirribosídeo, BUdR
• Fórmula empírica (Notação de Hill): C₉H₁₁BrN₂O₅
• Número CAS: 59-14-3
• Peso molecular: 307.10
• Beilstein: 30395
• Número MDL: MFCD00006529
• Código UNSPSC: 12352204
• ID de substância PubChem: 329749004

Propriedades

• Nível de qualidade: 100
• Ensaio: >97%
• forma: crystalline solid
• peso molecular: 307.1
• embalagem: pkg of 1 g
• fabricante/nome comercial: Roche
• pf: 191-194 °C (dec.) (lit.)
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• chave InChI: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Descrição

Descrição geral

It is conventionally used as a marker for S-phase synthesis of DNA.

Aplicação

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Ações bioquímicas/fisiológicas

Análogo de timidina usado como mutagênico em pesquisas genéticas. Incorporado seletivamente ao DNA celular durante a fase S.

Qualidade

Purity: 97% (from N), chromatographically homogeneous

Nota de preparo

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H340,H361fd
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Muta. 1B - Repr. 2
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable