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A8580

Sigma-Aldrich

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Sinônimo(s):

Anti-Glutathione-S-Transferase, Anti-GST

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

rabbit

Nível de qualidade

conjugado

agarose conjugate

forma do anticorpo

IgG fraction of antiserum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

PBS suspension

peso molecular

antigen 27.5 kDa

não deve reagir com

porcine, bovine, human, rabbit, rat

técnica(s)

ELISA: suitable
immunoprecipitation (IP): suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GSTP1(2950)

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Descrição geral

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Imunogênio

recombinant GST from Schistosoma japonicum expressed in E. coli.

Aplicação

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.
Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Ações bioquímicas/fisiológicas

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

forma física

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Nota de preparo

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Metabolism of xenobiotics of human environments
Progress in Molecular Biology and Translational Science, 112, 31-88 (2012)
Clinical pathology in non-clinical toxicology testing
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The role of glutathione-S-transferase in anti-cancer drug resistance
Townsend D M and Tew K D
Oncogene, 22(47), 7369-7369 (2003)
Xun Shang et al.
The Journal of biological chemistry, 282(12), 8801-8811 (2007-02-03)
p200 RhoGAP, a member of the Rho GTPase-activating protein (RhoGAP) family, was previously implicated in the regulation of neurite outgrowth through its RhoGAP activity. Here we show that ectopic expression of p200 RhoGAP stimulates fibroblast cell proliferation and cell cycle

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