A7345
Anti-V5 Agarose Affinity Gel antibody produced in mouse
purified immunoglobulin, clone V5-10
Sinônimo(s):
Monoclonal Anti-V5
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
agarose conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
V5-10, monoclonal
classe(s) química(is) do analito
proteins (V5)
técnica(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
capacidade
2.5 nmol/mL, gel (V5 fusion protein)
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Procurando produtos similares? Visita Guia de comparação de produtos
Descrição geral
Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.
Especificidade
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Aplicação
Anti-V5 Agarose Affinity Gel antibody produced in mouse has been used in immunoprecipitation and recombinant protein purification.
Ações bioquímicas/fisiológicas
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest. The addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag. Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.
forma física
Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.
Armazenamento e estabilidade
For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.
Exoneração de responsabilidade
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Arko Dasgupta et al.
PLoS genetics, 11(5), e1005215-e1005215 (2015-05-16)
Most organisms on earth sense light through the use of chromophore-bearing photoreceptive proteins with distinct and characteristic photocycle lengths, yet the biological significance of this adduct decay length is neither understood nor has been tested. In the filamentous fungus Neurospora
Q Meng et al.
Analytical biochemistry, 421(2), 351-361 (2011-11-01)
Quantitation of individual monoclonal antibodies (mAbs) within a combined antibody drug product is required for preclinical and clinical drug development, including pharmacokinetic (PK), toxicology, stability, and biochemical characterization studies of such drugs. We have developed an antitoxin, XOMA 3AB, consisting
Birger Herzog et al.
Molecular biology of the cell, 22(15), 2766-2776 (2011-06-10)
In endothelial cells, neuropilin-1 (NRP1) binds vascular endothelial growth factor (VEGF)-A and is thought to act as a coreceptor for kinase insert domain-containing receptor (KDR) by associating with KDR and enhancing VEGF signaling. Here we report mutations in the NRP1
Mariana Fernandez-Caggiano et al.
The Journal of biological chemistry, 291(19), 10399-10410 (2016-03-06)
The role and responses of the dimeric DJ-1 protein to cardiac oxidative stress is incompletely understood. H2O2 induces a 50-kDa DJ-1 interprotein homodimer disulfide, known to form between Cys-53 on each subunit. A trimeric 75-kDa DJ-1 complex that mass spectrometry
Cynthia Dayer et al.
The Journal of biological chemistry, 290(22), 13763-13778 (2015-04-01)
Solid tumor growth triggers a wound healing response. Similar to wound healing, fibroblasts in the tumor stroma differentiate into myofibroblasts (also referred to as cancer-associated fibroblasts) primarily, but not exclusively, in response to transforming growth factor-β (TGF-β). Myofibroblasts in turn
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica