A274
Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse
clone 2G11, ascites fluid, buffered aqueous solution
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About This Item
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
2G11, monoclonal
Formulário
buffered aqueous solution
peso molecular
antigen 60-80 kDa (glycosylated form)
reatividade de espécies
pig, mouse, rabbit, rat, bovine, ferret, canine
técnica(s)
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:2,000
western blot (chemiluminescent): 1:4,000
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
mouse ... Atp4b(11945)
rat ... Atp4b(24217)
Descrição geral
The H+/K+-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H+/K+-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H+/K+ ATPase (β subunit) binds to H+/K+-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.
The antibody reacts with various forms of the H+/K+ ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H+/K+-ATPase and to alter the affinity of the cytoplasmic K+ binding site. It may be used to localize and detect H+/K+ ATPase β subunit.
Imunogênio
core 34 kDa H+/K+ ATPase β subunit from deglycosylated pig gastric microsomes.
Aplicação
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Mouse monoclonal anti-H+/K+ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.
forma física
Solution in phosphate buffered saline containing 0.05% sodium azide.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Ivana Novak et al.
The Journal of biological chemistry, 286(1), 280-289 (2010-10-28)
Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma
Salim M A Bastaki et al.
Molecular and cellular biochemistry, 341(1-2), 43-50 (2010-03-20)
Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats
D C Chow et al.
The American journal of physiology, 265(6 Pt 1), C1562-C1570 (1993-12-01)
The gastric proton pump, H(+)-K(+)-ATPase, is composed of alpha- and beta-subunits. The 95-kDa alpha-subunit has been referred to as the catalytic subunit containing sites for ATP binding and phosphorylation. The beta-subunit is a glycoprotein with a 34-kDa core peptide that
Marco Tozzi et al.
Cancers, 12(3) (2020-03-14)
Pancreatic duct cells are equipped with acid/base transporters important for exocrine secretion. Pancreatic ductal adenocarcinoma (PDAC) cells may utilize such transporters to acidify extracellular tumor microenvironment, creating a niche favoring cell proliferation, fibrosis and resistance to chemotherapy-all contributing to the
Jing Wang et al.
PloS one, 10(5), e0126432-e0126432 (2015-05-21)
The mechanism by which pancreas secretes high HCO3- has not been fully resolved. This alkaline secretion, formed in pancreatic ducts, can be achieved by transporting HCO3- from serosa to mucosa or by moving H+ in the opposite direction. The aim
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