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Documentos Principais

A2289

Sigma-Aldrich

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

Sinônimo(s):

TRITC-albumin

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About This Item

Número CAS:
9048-46-8
Número MDL:
MFCD00130416
Código UNSPSC:
12352202
NACRES:
NA.25

fonte biológica

bovine

Nível de qualidade

200

Ensaio

≥90% (Lowry)

forma

lyophilized powder

Extensão da rotulagem

≥0.5 mol TRITC per mol albumin

técnica(s)

cell culture | mammalian: suitable

Impurezas

salt, free

nº de adesão UniProt

P02769

temperatura de armazenamento

2-8°C

Informações sobre genes

bovine ... ALB(280717)

Descrição geral

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Aplicação

Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Nota de preparo

Prepared from crystallized and lyophilized albumin (A4378).

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análise (COA)

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Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
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