A0157
Ascorbate Oxidase from Cucurbita sp.
lyophilized powder, 1,000-3,000 units/mg protein
Sinônimo(s):
L-Ascorbate:oxygen oxidoreductase, Ascorbase
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About This Item
Produtos recomendados
fonte biológica
plant (Cucurbita spp.)
Nível de qualidade
forma
lyophilized powder
atividade específica
1,000-3,000 units/mg protein
temperatura de armazenamento
2-8°C
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Descrição geral
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Aplicação
Ascorbate Oxidase from Cucurbita sp. has been used:
- as a supplement in the culture medium for differentiation into osteoblasts
- to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
- in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .
Ações bioquímicas/fisiológicas
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Definição da unidade
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
forma física
Lyophilized powder containing buffers and sucrose as stabilizer.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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