94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Sinônimo(s): Urate oxidase
• Número CAS: 9002-12-4
• Número da licença da enzima: 1.7.3.3 (BRENDA, IUBMB)
• Número MDL: MFCD00082126
• Código UNSPSC: 12352204
• ID de substância PubChem: 57653596
• NACRES: NA.54

Propriedades

• fonte biológica: Bacillus sp. (B. fastidiosus)
• Nível de qualidade: 300
• Formulário: powder
• qualidade: lyophilized
• atividade específica: ~9 U/mg
• condição de armazenamento: dry at room temperature
• concentração: 90—100%
• cor: white to faint beige
• solubilidade: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• aplicação(ões): detection
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• chave InChI: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Descrição

Descrição geral

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Aplicação

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Ações bioquímicas/fisiológicas

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Definição da unidade

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Outras notas

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H319,H334,H360FD
• Declarações de precaução: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)