93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Sinônimo(s): Monolaurato de polietilenoglicol sorbitano, Monolaurato de polioxietileno sorbitano
• Número CAS: 9005-64-5
• Número MDL: MFCD00165986
• Código UNSPSC: 12161900
• NACRES: NA.21

Propriedades

• descrição: non-ionic
• Nível de qualidade: 200
• Formulário: viscous liquid
• peso molecular: ~1228
• técnica(s): DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• índice de refração: n20/D 1.468 (lit.); n20/D 1.471
• viscosidade: 250-450 mPa.s(25 °C)
• Valor HLB: 16.7±1.0
• densidade: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• cadeia de caracteres SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• chave InChI: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Descrição

Descrição geral

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Aplicação

Tween^® 20 is widely used in several biochemical applications. It has been used:

• as an emulsifying agent for the preparation of stable oil-in-water emulsions
• in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• for rehydrating tissue before in situ hybridization

Detergente não iônico

Ações bioquímicas/fisiológicas

Tween^® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Características e benefícios

• Suitable for protein purification and DNA and RNA extraction
• Suitable for mammalian cell culture
• High purity product for research applications

Atenção

may get turbid on storage, heat to clear

Outras notas

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Informações legais

TWEEN is a registered trademark of Croda International PLC

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 527.0 °F - Pensky-Martens closed cup
• Ponto de fulgor (°C): 275 °C - Pensky-Martens closed cup
• Equipamento de proteção individual: Eyeshields, Gloves