P2287

TWEEN^® 20

viscous liquid, suitable for cell culture

• Sinônimo(s): Monolaurato de polietilenoglicol sorbitano, Monolaurato de polioxietileno sorbitano
• Número CAS: 9005-64-5
• Número MDL: MFCD00165986
• Código UNSPSC: 12161900
• NACRES: NA.21

Propriedades

• descrição: non-ionic
• Nível de qualidade: 300
• Formulário: viscous liquid
• peso molecular: ~1228
• composição: lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
• concentração: ≥40.0%
• técnica(s): cell culture | mammalian: suitable
• índice de refração: n20/D 1.468 (lit.)
• CMC: 0.06 mM (20-25°C)
• temperatura de transição: cloud point 76 °C
• solubilidade: water: soluble
• densidade: 1.095 g/mL at 25 °C (lit.); 1.1 g/cm³ at 25 °C (lit.)
• HLB: 16.7
• cadeia de caracteres SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• chave InChI: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Descrição

Descrição geral

Tween 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Aplicação

Detergente não iônico

TWEEN 20 has been used in staining buffer for in situ hybridization. It has also been used to prevent non-specific adsorption of proteins to coverslip during sample preparation for FRET (Förster resonance energy transfer) experiments.

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Atenção

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Nota de preparo

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Informações legais

TWEEN is a registered trademark of Croda International PLC

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 527.0 °F - Pensky-Martens closed cup
• Ponto de fulgor (°C): 275 °C - Pensky-Martens closed cup
• Equipamento de proteção individual: Eyeshields, Gloves