59304

2-propanol

≥99.5% (GC), for molecular biology, BioUltra

• Sinônimo(s): Álcool sec-propílico, IPA, Isopropanol, Álcool isopropílico
• Fórmula linear: (CH₃)₂CHOH
• Número CAS: 67-63-0
• Peso molecular: 60.10
• Beilstein: 635639
• Número CE: 200-661-7
• Número MDL: MFCD00011674
• Código UNSPSC: 12352104
• ID de substância PubChem: 57651672
• NACRES: NA.25

Propriedades

• product name: 2-propanol, BioUltra, for molecular biology, ≥99.5% (GC)
• grau: for molecular biology
• Nível de qualidade: 200
• densidade de vapor: 2.1 (vs air)
• pressão de vapor: 33 mmHg ( 20 °C); 44 mmHg ( 25 °C)
• linha de produto: BioUltra
• Ensaio: ≥99.5% (GC)
• forma: liquid
• temperatura de autoignição: 750 °F
• Lim. expl.: 2.0-12.7 %, 93 °C
• técnica(s): DNA extraction: suitable; RNA extraction: suitable
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• resíduo de evaporação: ≤0.001%
• cor: colorless
• índice de refração: n20/D 1.377 (lit.); n20/D 1.378
• pb: 82 °C (lit.)
• pf: −89.5 °C (lit.)
• solubilidade: water: soluble (completely)
• densidade: 0.785 g/mL at 25 °C (lit.)
• traços de cátion: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤0.5 mg/kg; Ni: ≤0.02 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• cadeia de caracteres SMILES: CC(C)O
• λ: neat
• Absorção UV: λ: 260 nm A_max: 0.02; λ: 280 nm A_max: 0.01
• adequação: suitable for molecular biology
• InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
• chave InChI: KFZMGEQAYNKOFK-UHFFFAOYSA-N

Descrição

Descrição geral

2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.

Aplicação

2-Propanol has been used:

• to precipitate genomic DNA extracted from flies
• to precipitate genomic DNA extracted from xenograft primary tumors and organs
• to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)

Ações bioquímicas/fisiológicas

Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.

Características e benefícios

• Suitable as a reagent for Biochemical and Molecular Biology Research
• BioUltra grade, meets strict purity standards through rigorous testing
• Free from DNase, NICKase, RNase, and Protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Outras notas

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Informações de segurança

• Pictogramas: GHS02,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H225,H319,H336
• Declarações de precaução: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Órgãos-alvo: Central nervous system
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 53.6 °F - closed cup
• Ponto de fulgor (°C): 12.0 °C - closed cup
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter