108319

Cloridrato de Trizma^®

EMPROVE^® EVOLVE

• Sinônimo(s): Cloridrato de trometamina, TRIS, TRIS HCl, Tris(hidroximetil)-aminometano
• Fórmula linear: NH₂C(CH₂OH)₃ · HCl
• Número CAS: 1185-53-1
• Peso molecular: 157.60
• Beilstein: 3675235
• Número MDL: MFCD00012590
• Código UNSPSC: 12161700

Propriedades

• fonte biológica: synthetic
• Nível de qualidade: 400
• descrição: Manufactured under appropriate controls for use as a raw material in BioProcessing
• linha de produto: EMPROVE^® EVOLVE
• forma: powder
• técnica(s): cell culture | mammalian: suitable
• Impurezas: Endotoxin, tested; Microbial, tested; Trace Metals, tested
• faixa de pH útil: 7.0-9.0
• pKa (25 °C): 8.1
• adequação: suitable for manufacturing use
• atividade externa: Cytotoxicity, contains (tested); DNase, contains (tested); NICKase, contains (tested); Protease, contains (tested); RNase, contains (tested)
• cadeia de caracteres SMILES: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• chave InChI: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Descrição

Descrição geral

Our SAFC^® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

As part of our EMPROVE^® Program, our raw materials are offered with EMPROVE^® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Aplicação

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.

Outras notas

Os valores de pH de todos os tampões são dependentes da temperatura e da concentração. Para tampões Tris, o pH aumenta cerca de 0,03 unidade por °C de diminuição na temperatura e diminui 0,03-0,05 unidade por diluição de 10 vezes. Para aplicações precisas, use um medidor de pH cuidadosamente calibrado com eletrodo de combinação de vidro/calomelano.

Informações legais

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable