10112301
P4E6 cell line
human prostate, Epithelial
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About This Item
Código UNSPSC:
41106514
Produtos recomendados
product name
P4E6 cell line,
fonte biológica
human prostate
modo de crescimento
Not specified
cariótipo
Not specified
morfologia
Epithelial
produtos
Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).
receptores
Not specified
técnica(s)
cell culture | mammalian: suitable
doença(s) relevante(s)
cancer
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Origem de linhagem celular
Prostate cancer well differentiated, early stage
Perfil do DNA
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Meio de cultura
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Rotina de subcultura
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Outras notas
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. Additionally, any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com).
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
N J Maitland et al.
Radiation research, 155(1 Pt 2), 133-142 (2000-12-20)
In Vitro Models to Study Cellular Differentiation and Function in Human Prostate Cancers. To augment the currently available models of human prostate cancer in vitro, we have established extended life-span epithelial cultures from biopsies of well-differentiated prostate cancers. The genetic
Shona H Lang et al.
The Prostate, 52(4), 253-263 (2002-09-05)
The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers. Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines
Shona H Lang et al.
In vitro cellular & developmental biology. Animal, 42(8-9), 273-280 (2006-12-14)
Three-dimensional epithelial culture models are widely used to emulate a more physiologically relevant microenvironment for the study of genes and signaling pathways. Prostate epithelial cells can grow into solid cell masses or acinus-like spheroids in Matrigel. To test if the
Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene.
Norman J Maitland et al.
Methods in molecular medicine, 88, 275-285 (2003-11-25)
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