PNT2 (SERUM FREE)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human prostate
• embalagem: tube of 5 μg 07042701-DNA-5UG; pkg of vial of cells 07042701-1VL
• modo de crescimento: Adherent
• cariótipo: Not
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human prostate normal, serum-free

Descrição de linhagem celular

This cell line is a derivative of the normal human prostate cell line PNT2 (Sigma cat no. 95012613) adapted to grow in serum-free medium. PNT2 was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein.

Meio de cultura

Defined Keratinocyte-SFM (Gibco 10785) + Growth Supplement (Gibco 10784-015).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Use Trypsin Inhibitor to inactivate the trypsin. When freezing cells down use conditioned medium plus 10% DMSO.

Outras notas

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