W0625

Wright stain

≤4% purity (HPLC), powder

• Sinônimo(s): Eosin Methylene blue according to Wright
• Número CAS: 68988-92-1
• Número MDL: MFCD00082143
• Código UNSPSC: 12171500
• NACRES: NA.47

Propriedades

• Nome do produto: Wright stain, suitable for blood stain
• Ensaio: ≤4% (HPLC)
• Nível de qualidade: 200
• Formulário: powder
• concentração: ≤4% (HPLC)
• técnica(s): microbe id | staining: suitable
• cor: dark green
• solubilidade: methanol: 10 mg/10 mL
• adequação: suitable for blood stain
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• chave InChI: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Descrição

Descrição geral

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Aplicação

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Características e benefícios

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Nota de preparo

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302,H319
• Declarações de precaução: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Classificações de perigo: Acute Tox. 4 Oral - Eye Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves