PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Sinônimo(s): 10X TE buffer
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• linha de produto: pHast Pack^™
• forma: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 7.9—8.1
• solubilidade: soluble, clear, colorless
• traços de cátion: Fe: <10 ppm
• atividade externa: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Aplicação

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Características e benefícios

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouch

Reconstituição

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Warning
• Frases de perigo: H373
• Declarações de precaução: P260 - P314 - P501
• Classificações de perigo: STOT RE 2 Inhalation
• Órgãos-alvo: Respiratory Tract
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable