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MBD0013

Sigma-Aldrich

Microbial DNA standard from Escherichia coli

Suitable for PCR, sequencing and NGS, 10 ng/μL

Sinônimo(s):

E.Coli

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About This Item

Código UNSPSC:
41105500
NACRES:
NA.51

Nível de qualidade

forma

liquid

concentração

10 ng/μL

técnica(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

adequação

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Descrição geral

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.


Read here how to use our standards to ensure data integrity for your microbiome research.

Aplicação

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Características e benefícios

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

forma física

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Outras notas

Strain: MG1655

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Order and disorder during Escherichia coli divergence.
Heather Hendrickson
PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
Lisa C Crossman et al.
Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
James B Kaper et al.
Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

Artigos

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

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