Pular para o conteúdo
Merck
Todas as fotos(2)

Key Documents

View All Documentation

E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Sinônimo(s):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Fórmula linear:
[-CH2N(CH2CO2Na)CH2CO2H]2
Número CAS:
139-33-3
Peso molecular:
336.21
Beilstein:
3822669
Número MDL:
MFCD00070672
Código UNSPSC:
12352107
ID de substância PubChem:
24894660
NACRES:
NA.31

grau

for molecular biology

Nível de qualidade

300

esterilidade

0.2 μm filtered

forma

liquid

adequação da reação

reagent type: chelator

concentração

0.5 M in H2O

pH

7.5-8.5

adequação

suitable for molecular biology

aplicação(ões)

microbiology

atividade externa

DNase, RNase, NICKase and protease, none detected

cadeia de caracteres SMILES

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

chave InChI

ZGTMUACCHSMWAC-UHFFFAOYSA-L

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Aplicação

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Características e benefícios

molecular biology grade

Nota de preparo

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Warning

Frases de perigo

H373

Declarações de precaução

P260 - P314 - P501

Classificações de perigo

STOT RE 2 Inhalation

Órgãos-alvo

Respiratory Tract

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SLCL5409
BCCG6590
BCCG1836
BCCG0945
BCCF9230

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 4

1 of 4

Ácido etilenodiamino tetra-acético Sigma Grade, suitable for plant cell culture, 98.5-101.5%

Sigma-Aldrich

E6635

Ácido etilenodiamino tetra-acético

Trizma® hydrochloride solution pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture

Sigma-Aldrich

T2694

Trizma® hydrochloride solution

Ácido etilenodiamino tetra-acético 98.5-101.5%, BioUltra

Sigma-Aldrich

E1644

Ácido etilenodiamino tetra-acético

Ethylenediaminetetraacetic acid BioUltra, ≥99.0% (KT)

Sigma-Aldrich

03609

Ethylenediaminetetraacetic acid

Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Jarrod S Johnson et al.
Cell host & microbe, 23(3), 366-381 (2018-03-16)
Myeloid dendritic cells (DCs) have the innate capacity to sense pathogens and orchestrate immune responses. However, DCs do not mount efficient immune responses to HIV-1, primarily due to restriction of virus reverse transcription, which prevents accumulation of viral cDNA and
Yongchao Dou et al.
Cell, 180(4), 729-748 (2020-02-16)
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Joshua B Black et al.
Cell reports, 33(9), 108460-108460 (2020-12-03)
Technologies to reprogram cell-type specification have revolutionized the fields of regenerative medicine and disease modeling. Currently, the selection of fate-determining factors for cell reprogramming applications is typically a laborious and low-throughput process. Therefore, we use high-throughput pooled CRISPR activation (CRISPRa)
Exibir todos os documentos relacionados

Protocolos

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica