D9564

DAPI, dilactate

≥98% purity (HPLC), powder

• Sinônimo(s): 4′,6-Diamidino-2-phenyindole, dilactate
• Fórmula empírica (Notação de Hill): C₁₆H₁₅N₅ · 2C₃H₆O₃
• Peso molecular: 457.48
• Código UNSPSC: 12171500
• ID de substância PubChem: 329798905
• NACRES: NA.47

Propriedades

• product name: DAPI, dilactate, ≥98% (HPLC)
• Nível de qualidade: 300
• Ensaio: ≥98% (HPLC)
• forma: powder
• cor: yellow
• solubilidade: H₂O: 20 mg/mL
• ε (coeficiente de extinção): 28.00-32.50 at 345-351 nm in methanol
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
• chave InChI: LCAUPANIXBMNIA-UHFFFAOYSA-N

Descrição

Descrição geral

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Aplicação

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Ações bioquímicas/fisiológicas

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)