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51490

Millipore

Hektoen Enteric Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

Código UNSPSC:
41171606
NACRES:
NA.74

esterilidade

non-sterile

Nível de qualidade

200

forma

powder

prazo de validade

limited shelf life, expiry date on the label

composição

acid fuchsin, 0.1 g/L
agar, 15.0 g/L
ammonium ferric citrate, 1.5 g/L
bile salts, 9 g/L
bromothymol blue, 0.065 g/L
lactose, 12 g/L
mixed peptone, 12 g/L
salicin, 2 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 5 g/L
sucrose, 12 g/L
yeast extract, 3 g/L

fabricante/nome comercial

NutriSelect® Plus

técnica(s)

microbiological culture: suitable

pH final

7.5±0.2 (25 °C)

aplicação(ões)

clinical testing
environmental
food and beverages
water monitoring
microbiology

adequação

selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for bacteria (General Media)

Aplicação

Hektoen enteric agar is a type of primary standard media, which may be commonly used in the selective detection and isolation of Salmonella in food microbiology labs and in human stool specimens.

Nota de preparo

Suspend 76.67 g in 1 litre distilled water and let it soak. Heat up by constant stirring until boiling. Cool to 55 - 60 °C and pour into sterile plates. Do not autoclave. The Medium is very thermolabile and thus overheating should be avoided.

Outras notas

Plating medium for the isolation of enteric pathogens; Isolation of Shigellae

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H317

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análise (COA)

Lot/Batch Number
BCCG1674
BCCF4105
BCCD6192
BCCD4843
BCCD4842

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Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools
Perez.MJ, et al.
Journal of Clinical Microbiology, 41, 1130-1134 (2003)
S King et al.
Applied microbiology, 16(4), 579-581 (1968-04-01)
During this study, 2,855 stool specimens from patients at Cook County Hospital were cultured for enteric pathogens. Hektoen Enteric Agar (HE) was compared with E M B and S S Agars by replicate samplings with both direct and indirect methods.
Entis P
Food Microbiology: The Laboratory (2002)
W I Taylor et al.
Applied microbiology, 21(1), 32-37 (1971-01-01)
Two enrichment broths and four plating media were compared for efficiency of detection of enteric pathogens from 1,597 stool specimens. Of 170 salmonellae isolated from the composite of all methods, direct streaking yielded but 54%, whereas enrichment in gram-negative broth
S. King et al.
Applied Microbiology, 16, 557-557 (1968)

Artigos

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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