32211-M

Clorofórmio

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

• Sinônimo(s): Tricloreto de metilidino, Triclorometano
• Fórmula empírica (Notação de Hill): CHCl₃
• Número CAS: 67-66-3
• Peso molecular: 119.38
• Beilstein: 1731042
• Número CE: 200-663-8
• Número MDL: MFCD00000826
• Código UNSPSC: 12191502
• ID de substância PubChem: 329754102
• NACRES: NA.21
• Ensaio: 99.0-99.4% (GC)
• técnica(s): RNA extraction: suitable
• p.e.: 60.5-61.5 °C (lit.)
• pressão de vapor: 160 mmHg ( 20 °C)

Propriedades

• Agency: USP/NF; reag. ISO; reag. Ph. Eur.
• densidade de vapor: 4.1 (vs air)
• pressão de vapor: 160 mmHg ( 20 °C)
• grau: puriss. p.a.
• Ensaio: 99.0-99.4% (GC)
• Formulário: liquid
• contém: ~1% ethanol as stabilizer
• técnica(s): RNA extraction: suitable
• Impurezas: ≤0.00001% free chlorine (Cl); ≤0.00005% free acid (as HCl); ≤0.0005% non-volatile matter; ≤0.005% aldehydes and ketones (as CH₃COCH₃); ≤0.005% carbonyl compounds (as CO); ≤0.01% tetrachloroethene (GC); ≤0.01% tetrachloromethane (GC); ≤0.01% trichloroethene (GC); ≤0.01% water (Karl Fischer); ≤0.03% dichloromethane (GC); 0.6-1.0% ethanol (GC)
• índice de refração: n20/D 1.445 (lit.)
• p.e.: 60.5-61.5 °C (lit.)
• pf: −63 °C (lit.)
• densidade: 1.476-1.483 g/mL at 20 °C; 1.492 g/mL at 25 °C (lit.)
• traços de ânion: chloride (Cl^-): ≤0.0001%
• traços de cátion: Al: ≤0.50 ppm; B: ≤0.02 ppm; Ba: ≤0.10 ppm; Ca: ≤0.50 ppm; Cd: ≤0.05 ppm; Co: ≤0.02 ppm; Cr: ≤0.02 ppm; Cu: ≤0.02 ppm; Fe: ≤0.10 ppm; Mg: ≤0.10 ppm; Mn: ≤0.02 ppm; Ni: ≤0.02 ppm; Pb: ≤0.05 ppm; Sn: ≤0.10 ppm; Zn: ≤0.10 ppm
• cadeia de caracteres SMILES: ClC(Cl)Cl
• adequação: complies for appearance; complies for reaction against H₂SO₄; complies for suitability of determ. w. dithizone
• InChI: 1S/CHCl3/c2-1(3)4/h1H
• chave InChI: HEDRZPFGACZZDS-UHFFFAOYSA-N

Descrição

Descrição geral

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO₂ aqueous suspensions (wavelength range of 310-380nm).

Aplicação

Chloroform may be employed as a solvent for the following studies:

• Extraction of RNA from plant cells.
• Preparation of dioleoylphosphatidylcholine (DOPC) solution.
• Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).

It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.

Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.

Outras notas

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table

Informações de segurança

• Pictogramas: GHS06,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H302,H315,H319,H331,H336,H351,H361d,H372
• Declarações de precaução: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• Órgãos-alvo: Central nervous system, Liver,Kidney
• Código de classe de armazenamento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash