12158167001

Anti-HA-Biotin, High Affinity (3F10)

from rat IgG₁

• Sinônimo(s): antibody
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• conjugado: biotin conjugate
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 3F10, monoclonal
• Formulário: lyophilized (stabilized)
• embalagem: pkg of 50 μg
• fabricante/nome comercial: Roche
• Isotipo: IgG1
• sequência de epítopo: YPYDVPDYA
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.

Especificidade

Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Imunogênio

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Aplicação

Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:

• Dot blots
• ELISA (enzyme-linked immunosorbent assay)
• Western blots

Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.

Qualidade

Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.

Nota de preparo

Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:

• Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
• Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
• Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
• Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
• Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.

Working concentration: Working concentration of conjugate depends on application and substrate

The following concentrations should be taken as a guideline:

• Dot blot: 100 ng/ml
• ELISA: 100 ng/ml
• Western blot: 100 ng/ml

Reconstituição

Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317,H412
• Declarações de precaução: P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501
• Classificações de perigo: Aquatic Chronic 3 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash