11086090001

Calpain Inhibitor I

N-Acetyl-Leu-Leu-norleucinal, synthetic

• Sinônimo(s): Calpain Inhibitor I, n-acetyl-leu-leu-norleucinal, ALLN, Ac-LLnL-CHO, MG-101, N-Acetyl-L-leucyl-L-leucyl-L-norleucinal, N-Acetyl-Leu-Leu-Norleu-al
• Fórmula empírica (Notação de Hill): C₂₀H₃₇N₃O₄
• Número CAS: 110044-82-1
• Peso molecular: 383.53
• Beilstein: 7656053
• Número MDL: MFCD00065505
• Código UNSPSC: 12352204
• ID de substância PubChem: 329749147

Propriedades

• Nível de qualidade: 100
• Ensaio: 98% (chromatographically homogeneous)
• forma: powder
• peso molecular: M_r = 383.5
• embalagem: pkg of 25 mg
• fabricante/nome comercial: Roche
• pf: 182 °C
• solubilidade: DMF: 10 mg/mL; ethanol: 10 mg/mL; methanol: 10 mg/mL
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: [H]C(=O)[C@H](CCCC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O
• InChI: 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
• chave InChI: FMYKJLXRRQTBOR-BZSNNMDCSA-N

Descrição

Descrição geral

Calpain Inhibitor I is a strong, competitive inhibitor of the Ca²⁺-dependent neutral cysteine proteases calpain I and calpain II.

Especificidade

Calpain Inhibitor I is a strong, competitive inhibitor of the Ca²⁺-dependent neutral cysteine proteases calpain I (requiring 2–75 μM Ca²⁺ for activation) and calpain II (requiring 200–800 μM) with a K_i-value between 0.12 μM and 0.23 μM. Calpain II is inhibited to a lesser extent than calpain I. Calpain Inhibitor I is also a strong inhibitor of papain and cathepsin B and L. It shows only weak inhibition of cathepsin H and α-chymotrypsin and does not inhibit trypsin. ID₅₀ for 0.02 U platelet calpain is 0.05 μM.

Aplicação

Calpain inhibitor I inhibits calpain, which activates myosin light chain kinase and protein kinase C by partial proteolysis. The inhibitor should be membrane permeable due to low molecular weight and lack of charged residues.
Calpain Inhibitor I is used for western blotting methods.

Qualidade

Purity: 98% (from C); chromatographically homogeneous

Variante da fórmula

C₂₀H₃₇N₃O₄

Nota de preparo

Working concentration: 0.1 - 10 μM
The suggested starting concentration is 17 μg/ml. This is the concentration at which half maximal inhibition of calpain I is observed.
Working solution: Recommended solvent is DMF, ethanol, or methanol up to 10 mg/ml.
Storage conditions (working solution): -15 to -25 °C
Solutions in DMF, methanol, ethanol are stable for 4 weeks at -15 to -25 °C.

Reconstituição

Soluble in DMF, ethanol, or methanol to 10 mg/ml. For a stock solution, Roche recommends dissolving 1 mg of the inhibitor in 100 μl DMF, methanol, or ethanol. Before use, dilute with water or phosphate buffer (0.1 M, pH 7.5) to desired concentration. Solutions in DMF, ethanol, or methanol are stable for 2 to 3 days at 2 to 8 °C, and approximately 4 weeks at -15 to -25 °C. For best results, prepare solutions fresh before use.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable