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Key Documents

11051440001

Roche

dATP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

Sinônimo(s):

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, deoxy-adenosine-5′-triphosphate, 2′-, dATP

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About This Item

Código UNSPSC:
41116100

Nível de qualidade

descrição

Lithium salt

Ensaio

98%

forma

solution

uso

sufficient for 6,250 standard reactions (PCR)

embalagem

pkg of 250 μL (25 μmol; 100 mM)

fabricante/nome comercial

Roche

concentração

100 mM

técnica(s)

DNA amplification: suitable
DNA sequencing: suitable
DNA synthesis: suitable
PCR: suitable

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

chave InChI

YJWCICGGRLOGEH-VWZUFWLJSA-M

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Descrição geral

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.
dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Aplicação

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
dATP has been used to resume transcription.

Ações bioquímicas/fisiológicas

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

Qualidade

Typical analysis:
  • ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Definição da unidade

1 μmol dATP = 15.0 A260 units

Outras notas

For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism
Le TT, et al.
Cell (2018)
Stable suppression of the R2 subunit of ribonucleotide reductase by R2-targeted short interference RNA sensitizes p53(-/-) HCT-116 colon cancer cells to DNA-damaging agents and ribonucleotide reductase inhibitors
Lin ZP, et al.
The Journal of Biological Chemistry, 279(26), 27030-27038 (2004)
Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
Liu X, et al.
Cell, 86(1), 147-157 (1996)

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