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Documentos Principais

10109134001

Roche

RNase

from bovine pancreas

Sinônimo(s):

Rnase

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About This Item

Código UNSPSC:
12352204

fonte biológica

bovine pancreas

Nível de qualidade

Formulário

dry powder (prepared from crystallized ribonuclease)

atividade específica

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

embalagem

pkg of 500 mg

fabricante/nome comercial

Roche

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Especificidade

Heat inactivation: 1 mM EDTA

Aplicação

RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

Definição da unidade

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Nota de preparo

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


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