SCT246

BioTracker BioCyTASQ G-Quadruplex (G4) Cell Probe

• Sinônimo(s): G quadruplex probe, G4 ligand, G4 probe, G4 tetrad probe, Live Cell Imaging Probe
• Código UNSPSC: 41106514
• NACRES: NA.47

Propriedades

• embalagem: vial of 1 mg
• Nível de qualidade: 100
• fabricante/nome comercial: Millipore
• modo de crescimento: N/A
• técnica(s): DNA sequencing: suitable; RNA extraction: suitable; nucleic acid detection: suitable
• método de detecção: fluorometric
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Aplicação

DNA/RNA G-quadruplexes (G4s) are four-stranded nucleic acid structures that fold from numerous guanine (G)-rich sequences found in the genome and transcriptome. Their abundance and localization in critical regulatory regions make them key players in genetic diseases (cancer, neuropathology), but not only (e.g., viral infection). For this reason G4s have garnered intense interest as potential targets in different therapeutic areas.


The BioTracker^™ BioCyTASQ G-quadruplex G4 cell probe is optimized for detection (1) and/or the isolation of G4s (2,3). BioCyTASQ utilizes the template-assembled synthetic G-quartets (TASQ) (4) concept as a biomimetic ligand for G-quadruplexes, a design which results in high specificity for folded G4.

References1.ACS Chem Biol. 2021, 16: 905–914 2. Nature Protoc. 2022, 17: 870–889 3. STAR Protoc. 2024, 5: 103480 4. Acc. Chem. Res. 2023, 56: 350–362 5. iScience 2023, 26:106846

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis

Características e benefícios

BioCyTASQ is suited to visualize G4s in cells in a 2-step protocol referred to as pre-targeted G4 imaging, which relies on the use of a fluorescent streptavidin (1). BioCyTASQ is also suited to isolate G4s by affinity capture experiments, in a protocol named G4RP for RNA G4s, (2,3) or G4DP for DNA G4s (5)). These G4-selective chemoprecipitations allow for their identification by either PCR, RT-PCR, or sequencing.

forma física

Lyophilized. White solid.

Armazenamento e estabilidade

Store BioTracker BioCyTASQ G-quadruplex G4 cell probe at -20°C, desiccated and protected from light

Outras notas

Fluorescent detection of nucleic acid G4 quadruplexes in cells and tissues

Informações legais

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable