SCC117
Histone H2B-GFP expressing HeLa Cell Line
Human
Sinônimo(s):
Histone H2B
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About This Item
Código UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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Nome do produto
Histone H2B-GFP expressing HeLa Cell Line, The Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics.
fonte biológica
human
Nível de qualidade
técnica(s)
cell based assay: suitable
cell culture | mammalian: suitable
Condições de expedição
ambient
Descrição geral
Chromatin, the higher order structure of DNA, proteins and RNA, constitutes the majority of the nucleus of eukaryotic cells. Changes in chromatin structure are the essence of many essential nuclear processes, including transcription, meiosis, mitosis, and apoptosis. The nucleosome represents the primary building block of chromatin and it comprises an octomer of four core histone proteins (H2A, H2B, H3 and H4). HeLa cells expressing green fluorescent protein fused histone H2B (H2B-GFP) have been widely used to visualize the dynamics of chromosomal architecture in living cells during various processes. In addition to studying normal mitosis, histone H2B-GFP has been utilized for imaging the distinctive clustering behavior of double minute chromosomes (DMs) in cancer cells during mitosis, which contributes to their asymmetric distribution to daughter cells. Monitoring histone H2B-GFP also permits continuous analysis of chromosomal degradation during apoptosis.
Descrição de linhagem celular
Cancer Cells
Aplicação
Research Category
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Chromatin Biology
Histones
Cell Cycle, DNA Replication & Repair
Chromatin Biology
Histones
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Qualidade
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Armazenamento e estabilidade
Histone H2B-GFP expressing HeLa Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Oral
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
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J L Workman et al.
Annual review of biochemistry, 67, 545-579 (1998-10-06)
The nucleosome, which is the primary building block of chromatin, is not a static structure: It can adopt alternative conformations. Changes in solution conditions or changes in histone acetylation state cause nucleosomes and nucleosomal arrays to behave with altered biophysical
Athea Vichas et al.
Nature communications, 12(1), 4789-4789 (2021-08-11)
CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred
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