QIA127

Rapid Cell Proliferation Kit

• Sinônimo(s): Proliferation assay kit
• Código UNSPSC: 12352207
• NACRES: NA.84

Propriedades

• uso: sufficient for 500 tests
• Nível de qualidade: 100
• embalagem: pkg of 1 96-well plate(s)
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• entrada: sample type cultured cells (1,000-50,000 cells)
• método de detecção: colorimetric
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

Características e benefícios

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

Componentes

WST-1, 1-Methoxy PMS, and a user protocol.

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Especificações

Assay Time: 0.5 - 4 h

Princípio

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

Nota de preparo

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

Outras notas

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids