NA03
Anti-PCNA (Ab-1) Mouse mAb (PC10)
liquid, clone PC10, Calbiochem®
Sinônimo(s):
Anti-Proliferating Cell Nuclear Antigen
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
PC10, monoclonal
Formulário
liquid
contém
≤0.1% sodium azide as preservative
reatividade de espécies
rat, yeast, mouse, human, zebrafish
fabricante/nome comercial
Calbiochem®
condição de armazenamento
do not freeze
Isotipo
IgG2a
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... PCNA(5111)
zebrafish ... Pcna(30678)
Descrição geral
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).
Imunogênio
Epitope: Within amino acids 112-121
recombinant PCNA
Aplicação
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Embalagem
Please refer to vial label for lot-specific concentration.
Advertência
Toxicity: Standard Handling (A)
Nota de análise
Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Outras notas
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
B K Law et al.
The Journal of biological chemistry, 275(49), 38261-38267 (2000-09-20)
Salicylate and its pro-drug form aspirin are widely used medicinally for their analgesic and anti-inflammatory properties, and more recently for their ability to protect against colon cancer and cardiovascular disease. Despite the wide use of salicylate, the mechanisms underlying its
Hailong Wang et al.
Journal of the American Heart Association, 8(14), e011994-e011994 (2019-07-13)
Background Exposure to chronic psychosocial stress is a risk factor for atherosclerosis-based cardiovascular disease. We previously demonstrated the increased expressions of cathepsin S (CatS) in atherosclerotic lesions. Whether CatS participates directly in stress-related neointimal hyperplasia has been unknown. Methods and
Mayu Inokuchi et al.
The Journal of experimental biology, 220(Pt 24), 4720-4732 (2017-11-01)
Spatiotemporal changes in branchial ionocyte distribution were investigated following transfer from seawater (SW) to freshwater (FW) in Japanese seabass. The mRNA expression levels of cystic fibrosis transmembrane conductance regulator (CFTR) and Na+/K+/2Cl- cotransporter 1a (NKCC1a) in the gills rapidly decreased
Arianna Casciati et al.
Oncotarget, 7(19), 28040-28058 (2016-04-09)
Therapeutic irradiation of pediatric and adult patients can profoundly affect adult neurogenesis, and cognitive impairment manifests as a deficit in hippocampal-dependent functions. Age plays a major role in susceptibility to radiation, and younger children are at higher risk of cognitive
Bo Liu et al.
Molecular medicine reports, 14(2), 1099-1106 (2016-06-10)
The aim of the present study was to investigate the expression of matrix metalloproteinase (MMP)9 and MMP2, and their potential roles in bone metastasis nests using a well-standardized model of breast cancer bone metastasis in nude mice. BALB/c nu/nu mice
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica