MABS270

Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3

clone 5A5-1F3, from mouse

• Sinônimo(s): Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform, PP2A B subunit isoform B′-epsilon, ; ; PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 5A5-1F3, monoclonal
• reatividade de espécies: human, mouse
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_006237
• nº de adesão UniProt: Q16537
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PTPA(5524)

Descrição

Descrição geral

Protein phosphatase 2A (PP2A) is a family of ubiquitous heterotrimeric proteins that catalyze the removal of phosphate groups from serine and threonine residues. The PP2A enzymes contain core structural (A) and catalytic (C) subunits and variable regulatory B subunits. The B subunits regulate their catalytic activity, as well as dictate substrate specificity and subcellular localization. Recent studies have suggested that B56 epsilon subunits regulate p53-mediated apoptotic pathways, and can induce or suppress apoptosis depending on the cellular context. PP2A enzymes are also regulated by posttranslational modifications and endogenous inhibitors.

Especificidade

This antibody is specific for the B56 regulatory subunit of Protein phosphatase 2A (PP2A).

Imunogênio

His-tagged recombinant protein corresponding to human PP2A reg. subunit B56 epsilon (PPP2R5E).

Aplicação

Research Category
Neuroscience

Apoptosis & Cancer

Research Sub Category
Kinases & Phosphatases

Apoptosis - Additional

This Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 is validated for use in Western Blotting, ICC, Immunoprecipitation for the detection of PP2A reg. subunit B56 epsilon (PPP2R5E).

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of U2OS cell lysate and mouse brain tissue lysate.

Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).

Qualidade

Evaluted by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.

Descrição-alvo

~50 kDa observed

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
NIH/3T3 cell lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable