539514

Protein Phosphatase, Lambda, Recombinant, E. coli

• Sinônimo(s): Protein Phosphatase, Lambda
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• Ensaio: ≥95% (SDS-PAGE)
• forma: liquid
• atividade específica: ≥400,000 units/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• atividade externa: Protease, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn²⁺-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.

Research Area: Cell Signaling
The protein phosphatase encoded by bacteriophage λ (λPP) is part of a family of Ser/Thr phosphatases (Ser/Thr PPases), which includes eukaryotic protein phosphatases 1 (PP1), 2A (PP2A), and 2B (calcineurin).

Aplicação

Protein Phosphatase, Lambda, Recombinant, E. coli is suitable for the detection of phospho-KRAS.

Ações bioquímicas/fisiológicas

Protein phosphatases play a crucial role in embryonic development, metabolic homeostasis, stress responses, cell cycle transitions, and numerous other vital biological processes.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.

forma física

In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

Nota de preparo

Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl₂, and 100 µg/ml BSA.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Zhuo, S., et al. 1993. J. Biol. Chem.268, 17754.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Cohen, P.T.W. and Cohen, P. 1989. Biochem. J.260, 931.

Informações legais

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable