P6244
Protein Tyrosine Phosphatase 1B human
recombinant, expressed in E. coli
Sinônimo(s):
PTP-1B
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
Formulário
solution
atividade específica
≥20 units/mg protein
peso molecular
37.4 kDa
concentração
≥0.5 mg/mL
nº de adesão UniProt
doença(s) relevante(s)
diabetes; obesity; cancer
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... PTPN1(5770)
Procurando produtos similares? Visita Guia de comparação de produtos
Categorias relacionadas
Descrição geral
Protein tyrosine phosphatase 1B human (PTP1B) is encoded by the gene PTPN1. In human chromosome, the gene PTPN1 is localized on 20q13.13. The protein has 37 kDa catalytic domain and the C-terminal hydrophobic residues targets the protein to the cytoplasmic side of the endoplasmic reticulum membrane.
Aplicação
Protein Tyrosine Phosphatase 1B (PTP1B) human has been used in treating cell lysate prior to immunoprecipitation and protein pull-down studies. It has also been used in PTP1B activity assay and also for the evaluation of inhibitors like betulinic acid (BA) and sodium orthovanadate (Na3VO4), and 6-chloro-3-formyl-7-methylchromone (CFM) on PTP1B.
Protein tyrosine phosphatase 1B (PTP1B) is a pharmaceutical target used to study the treatment of various diseases such as obesity, cancer and type II diabetes mellitus.
The inhibitory effect of 8-(4-chlorophenylthio)-cAMP and 8-(4-chlorophenylthio)-cGMP on protein tyrosine phosphatase-1B has been assayed.
Ações bioquímicas/fisiológicas
PTP-1B is a recombinant, non-transmembrane protein expressed in E. coli containing amino acid residues 1-322 of human PTP-1B. It has a molecular mass of 37.4 kDa. It is an abundant intracellular enzyme that is thought to act as a negative regulator of certain signaling pathways. It dephosphorylates tyrosine-phosphorylated proteins and peptides, and is usually localized in the cytostolic domain of the ER.
PTP1B dephosphorylates c-Src in several human breast cancer cell lines has a regulatory role for PTP1B in the control of c-Src kinase activity. PTP1B is regulates the cardiovascular effects of leptin. PTP1B controls the JAK/STAT signaling pathway by dephosphorylation of JAK2.
Polymorphism in the gene PTPN1 is associated with type 2 diabetes and obesity.
Definição da unidade
One unit will hydrolyze 1.0 μmole of a phosphopeptide, EGFR fragment 988-998, per minute at pH 7.2 at 30 °C
forma física
Supplied as a solution in 50 mM HEPES (pH 7.2), 1 mM EDTA, 5 mM DTT, and 0.05% NP-40
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and Cellular Biology, MCB-00194 (2016)
A colorimetric biosensor based on guanidinium recognition for the assay of protein tyrosine phosphatase 1B and its inhibitors
Lv J, et al.
New. J. Chem., 41(23), 14414-14419 (2017)
Adsorption between TC-stabilized AuNPs and the phosphate group: application of the PTP1B activity assay
Lv J, et al.
Analyst, 140(23), 8017-8022 (2015)
Analysis of common PTPN1 gene variants in type 2 diabetes, obesity and associated phenotypes in the French population
Cheyssac C, et al.
BMC Medical Genetics, 7(1), 44-44 (2006)
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and cellular biology, MCB-00194 (2016)
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica