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MABN380

Sigma-Aldrich

Anti-APP-C99 Antibody

mouse monoclonal, mC99(70-80)

Sinônimo(s):

Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nome do produto

Anti-APP-C99 Antibody, clone mC99(70–80), ascites fluid, clone mC99(70-80), from mouse

fonte biológica

mouse

Nível de qualidade

100

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

mC99(70-80), monoclonal

reatividade de espécies

human, mouse

técnica(s)

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

nº de adesão NCBI

NP_000475

nº de adesão UniProt

P05067

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... APP(351)

Descrição geral

Amyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer′s disease (AD). UP on intracerebroventricular injection of this antibody, A-beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN381, Anti-APP-C99, clone mC99(1-7) was raised against a slightly different region of the same APP-C99 protein.

Imunogênio

Recombinant protein corresponding to Human APP-C99.

Aplicação

Anti-APP-C99 Antibody, clone mC99(70–80) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, IHC & IF.
Immunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
(Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunofluoresence Analysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Qualidade

Evaluated by Western Blotting in DAPT treated HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.

Descrição-alvo

~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

forma física

Mouse monoclonal IgGκ ascites
Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Selective neutralization of APP-C99 with monoclonal antibodies reduces the production of Alzheimer's A? peptides.
Houacine, Jemila, et al.
Neurobiology of Aging, 33, 2704-2714 (2012)
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