MABE254

Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

clone 10F1, from mouse

• Sinônimo(s): HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: 10F1, monoclonal
• application: ChIP; IP; WB
• reatividade de espécies: human
• técnica(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 2

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 10F1, monoclonal
• reatividade de espécies: human
• técnica(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_036519
• nº de adesão UniProt: Q9UM07
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PADI4(23569)

Descrição

Descrição geral

Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.

Imunogênio

GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.

Aplicação

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.

Qualidade

Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates.

Descrição-alvo

~74 kDa observed

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
DMSO treated and untreated HL-60 cell lysates

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable