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Key Documents

MABC44

Sigma-Aldrich

Anti-LAMP-3 Antibody, clone 16H11.2

clone 16H11.2, from mouse

Sinônimo(s):

LAMP, CD208, LAMP-3, DC-loysosome-associated membrane glycoprotein, DC LAMP, DC-LAMP, Protein TSC403

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

16H11.2, monoclonal

reatividade de espécies

human

técnica(s)

immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG2bκ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... LAMP3(27074)

Descrição geral

Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.

Imunogênio

Recombinant protein corresponding to human LAMP-3.

Aplicação

Detect LAMP-3 using this Anti-LAMP-3 Antibody, clone 16H11.2 validated for use in WB, IH.
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected LAMP-3 in colorectal adenocarcinoma tissue.
Research Category
Cell Structure
Research Sub Category
Apoptosis - Additional

Organelle & Cell Markers

Qualidade

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-3 in MCF-7 cell lysate.

Descrição-alvo

~65 kDa observed.
The calculated molecular weight of this protein is 41 kDa, but has been observed at ~65-90 kDa due to glycosolation (de Saint-Vis, B., et al. (1998). Immunity. 9(3):325-336).

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
MCF-7 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Junya Nishimura et al.
Esophagus : official journal of the Japan Esophageal Society, 16(4), 333-344 (2019-04-11)
Dendritic cells (DCs) are the most potent antigen-presenting cells to induce cytotoxic T lymphocytes in the tumor environment. After acquiring antigens, DCs undergo maturation and their expression of MHC and co-stimulation molecules are enhanced, along with lysosome-associated membrane glycoprotein 3

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