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Documentos Principais

MAB88916

Sigma-Aldrich

Anti-Fibronectin Antibody

CHEMICON®, mouse monoclonal, 3E3

Sinônimo(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
3E3, monoclonal
application:
ELISA
ICC
IHC
WB
reatividade de espécies:
human
técnica(s):
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
6

Nome do produto

Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3, clone 3E3, Chemicon®, from mouse

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

3E3, monoclonal

reatividade de espécies

human

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... FN1(2335)

Especificidade

The antibody reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin (Pierschbacker et al., 1981) comprimising the cell attachment site (Pierschbacker et al., 1981; Pierschabacker et al., 1983, 1984; Hayman et al., 1985; Ruoslahti et al., 1986; Hynes, 1985). It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface (Pierschbacker et al., 1981). Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser (Pierschabacker et al., 1984; Hayman et al., 1985; Ruoslahti et al., 1986).

Imunogênio

Epitope: cell attachment fragment
Purified human-fibronectin from amnion liquid.

Aplicação

Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3 is an antibody against Fibronectin Frag for use in ELISA, WB, IC, IH.
Immunocytochemistry: (5-20 μg/mL) Immunohistochemistry: (5-20 μg/mL) (Not tested on paraffin sections) Immunoblotting: (5-20 μg-mL)

ELISA

Inactivation or inhibition studies in cell culture.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

forma física

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

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Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion.
Benjamin G Keselowsky, David M Collard, Andres J Garcia
Journal of Biomedical Materials Research Part A null
Mechanism(s) of increased vascular cell adhesion on nanostructured poly(lactic-co-glycolic acid) films.
Miller, Derick C, et al.
Journal of Biomedical Materials Research Part A, 73, 476-484 (2005)
Molecular biology of fibronectin.
Hynes, R
Annual Review of Cell Biology, 1, 67-90 (1985)
Angela Orecchia et al.
Journal of cell science, 116(Pt 17), 3479-3489 (2003-07-17)
Vascular endothelial growth factor receptor-1 (VEGFR-1) is a tyrosine kinase receptor for several growth factors of the VEGF family. Endothelial cells express a membrane-spanning form of VEGFR-1 and secrete a soluble variant of the receptor comprising only the extracellular region.
Tiffany M Tsang et al.
The Journal of biological chemistry, 287(20), 16759-16767 (2012-03-27)
The Yersinia pestis adhesin molecule Ail interacts with the extracellular matrix protein fibronectin (Fn) on host cells to facilitate efficient delivery of cytotoxic Yop proteins, a process essential for plague virulence. A number of bacterial pathogens are known to bind

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