Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

MAB5256X

Sigma-Aldrich

Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated

clone NE14, from mouse, ALEXA FLUOR 488

Sinônimo(s):

200 kDa neurofilament protein, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

ALEXA FLUOR 488

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

NE14, monoclonal

reatividade de espécies

human, rat, pig, porcine

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

Isotipo

IgG1

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... NEFH(4744)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Descrição geral

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Especificidade

Reactivity with other species has not been tested.
This antibody recognizes Neurofilament H.

Imunogênio

Epitope: Unknown
Purified neurofilament polypeptides (Debus et al., 1983).

Aplicação

Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC.
Immunohistochemistry:
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Qualidade

Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture.

Descrição-alvo

~ 200 kDa

forma física

Protein G Purified
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide.

Armazenamento e estabilidade

Stable for 6 months at 2-8ºC from date of receipt.

Nota de análise

Control
Rat Cortex Cells.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Sienna S Drake et al.
International journal of molecular sciences, 23(23) (2022-12-12)
Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease driven by inflammation and demyelination in the brain, spinal cord, and optic nerve. Optic neuritis, characterized by inflammation and demyelination of the optic nerve, is a symptom in many patients with
Sandeep Sheth et al.
Scientific reports, 9(1), 9571-9571 (2019-07-04)
Adenosine A1 receptors (A1AR) are well characterized for their role in cytoprotection. Previous studies have demonstrated the presence of these receptors in the cochlea where their activation were shown to suppress cisplatin-induced inflammatory response and the resulting ototoxicity. Inhibition of
S S Oh et al.
Neuroscience, 212, 201-213 (2012-04-21)
The contribution of oxidative stress to diabetic complications including neuropathy is widely known. Mitochondrial and cellular damage are associated with the overproduction of reactive oxygen species and decreased levels or function of the cellular antioxidant mitochondrial manganese superoxide dismutase (SOD2).
Hiromi Nakai-Shimoda et al.
iScience, 25(1), 103609-103609 (2022-01-11)
Glucose-responsive ATP-sensitive potassium channels (KATP) are expressed in a variety of tissues including nervous systems. The depolarization of the membrane potential induced by glucose may lead to hyperexcitability of neurons and induce excitotoxicity. However, the roles of KATP in the

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica