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Key Documents

MAB4400

Sigma-Aldrich

Anti-Renilla Luciferase Antibody, clone 5B11.2

ascites fluid, clone 5B11.2, Chemicon®

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

5B11.2, monoclonal

reatividade da espécie (prevista por homologia)

all

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Renilla luciferase is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Renilla luciferase is often used as a reporter gene in sudies of gene expression or interaction. The 36 kDa enzyme catalyzes oxidation of the substrate coelenterazine to coelenteramide, resulting in the production of visible light. Renilla luciferase is not the same as luciferase from fireflies (Photalis pyralis) nor does it recognize the same substrates, so both enzymes can be used simultaneously in the same assay.

Especificidade

Renilla luciferase

Imunogênio

Renilla luciferase GST fusion protein

Aplicação

Detect Renilla Luciferase using this Anti-Renilla Luciferase Antibody, clone 5B11.2 validated for use in ELISA, FUNC, NEUT & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot:
A previous lot of this antibody was used in WB.

EIA:
A previous lot of this antibody was used in ELISA.

Neutralization:
A prevous lot of this antibody was used in NEAT.

Optimal working dilutions must be determined by end user.

Descrição-alvo

36 kDa

forma física

Mouse monoclonal IgG1 Ascites.
Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Renilla

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC.
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Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output.
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