MAB349
Anti-Beta (β)-Amyloid antibody
CHEMICON®, mouse monoclonal, 1.D5
Sinônimo(s):
Alz-90
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
Nome do produto
Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5, clone 1.D5, Chemicon®, from mouse
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
1.D5, monoclonal
reatividade de espécies
human, rat
fabricante/nome comercial
Chemicon®
técnica(s)
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... APP(351)
Categorias relacionadas
Especificidade
Reacts with the natural amyloid precursor protein A4(695).
Imunogênio
Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].
Aplicação
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
forma física
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Armazenamento e estabilidade
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recomendado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Johnson, MW; Stoll, L; Rubio, A; Troncoso, J; Pletnikova, O; Fowler, DR; Li, L
Journal of Forensic Sciences null
Magdolna Pákáski et al.
Neurochemistry international, 47(3), 190-195 (2005-06-16)
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3,4-Methylenedioxymethamphetamine (MDMA), but not morphine, alters APP processing in the rat brain.
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The International Journal of Neuropsychopharmacology, 10, 183-190 (2007)
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