MAB3060
Anti-Paxillin Antibody, clone 349
clone 349, Chemicon®, from mouse
Sinônimo(s):
Anti-AW108311, Anti-AW123232, Anti-Pax
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
349, monoclonal
reatividade de espécies
rat, human, mouse, canine, chicken
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... PXN(5829)
rat ... Pxn(360820)
Especificidade
Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.
Imunogênio
Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.
Aplicação
Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Western blot: 1:1,000-1:10,000
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Ligação
Replaces: 04-581
forma física
Format: Purified
Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Nota de análise
Control
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Stylianou, P; Skourides, PA
Mechanisms of Development null
Contact between human bone marrow stromal cells and B lymphocytes enhances very late antigen-4/vascular cell adhesion molecule-1-independent tyrosine phosphorylation of focal adhesion kinase, paxillin, and ERK2 in stromal cells.
L J Jarvis, J E Maguire, T W LeBien
Blood null
F N Leeuwen et al.
The Journal of cell biology, 139(3), 797-807 (1997-11-14)
The invasion-inducing T-lymphoma invasion and metastasis 1 (Tiam1) protein functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac1. Differentiation-dependent expression of Tiam1 in the developing brain suggests a role for this GEF and its effector Rac1
Matrix nonenzymatic glycosylation leads to altered cellular phenotype and intracellular tyrosine phosphorylation.
Hasegawa, G, et al.
The Journal of Biological Chemistry, 270, 3278-3283 (1995)
Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton.
R M Ezzell, W H Goldmann, N Wang, N Parashurama, N Parasharama, D E Ingber, R M Ezzell et al.
Experimental Cell Research null
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