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Key Documents

MAB2019Z

Sigma-Aldrich

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free

clone 15F11, Chemicon®, from mouse

Sinônimo(s):

MAB2019

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

15F11, monoclonal

reatividade de espécies

human

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ITGAV(3685)

Especificidade

Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.

Imunogênio

Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

Aplicação

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.
Immunoprecipitation

Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.

Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

forma física

Format: Purified
Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Khine Kyaw Oo et al.
BMC cancer, 21(1), 65-65 (2021-01-16)
Chemoresistance is one of the main problems in treatment of cancer. Periostin (PN) is a stromal protein which is mostly secreted from cancer associated fibroblasts in the tumor microenvironment and can promote cancer progression including cell survival, metastasis, and chemoresistance.
Characterization of monoclonal antibodies against integrin alpha V beta 5.
Stuiver, I and Smith, J W
Hybridoma, 14, 545-550 (1995)
Divalent cations regulate the organization of integrins alpha v beta 3 and alpha v beta 5 on the cell surface.
Stuiver, I, et al.
Journal of Cellular Physiology, 168, 521-531 (1996)
Jim O'Prey et al.
Journal of virology, 82(12), 5933-5939 (2008-04-04)
Adenoviruses for gene or oncolytic therapy are under development. Notable among these strategies is adenoviral delivery of the tumor suppressor p53. Since all therapeutics have limitations in certain settings, we have undertaken retroviral suppressor screens to identify genes conferring resistance
L1 stimulation of human glioma cell motility correlates with FAK activation.
Yang, M; Li, Y; Chilukuri, K; Brady, OA; Boulos, MI; Kappes, JC; Galileo, DS
Journal of Neuro-Oncology null

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