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IPFL20200

Millipore

Immobilon® -FL PVDF Membrane

10 sheets, 20cm x 20 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Sinônimo(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

Código UNSPSC:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-FL PVDF Membrane, 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Materiais

PVDF membrane
plain filter
white filter

Nível de qualidade

400

Características

hydrophobic

fabricante/nome comercial

Immobilon®

técnica(s)

dot blot: suitable
western blot: suitable

filtro C × L

20 cm × 20 cm

tamanho de poro

0.45 μm pore size

capacidade

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibilidade

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

método de detecção

chemiluminescent
colorimetric
fluorometric

Condições de expedição

ambient

Descrição geral

The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Aplicação

Immobilon®-FL PVDF Membrane has been used in western blotting.

Características e benefícios

Immobilon®-FL Membrane:
  • Designed for optimal fluorescent immunodetection
  • Extremely low background improves the sensitivity of all fluorescence detection protocols
  • suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detection

Informações legais

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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