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Key Documents

GF142

Sigma-Aldrich

Platelet Derived Growth Factor-AA human

>98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, suitable for cell culture

Sinônimo(s):

PDGF-AA

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Platelet Derived Growth Factor-AA Protein, Recombinant human, The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

fonte biológica

human

Nível de qualidade

Ensaio

>98% (SDS-PAGE and HPLC)

técnica(s)

cell culture | mammalian: suitable

entrada

sample type neural stem cell(s)

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

Informações sobre genes

human ... PDGFA(5154)

Descrição geral

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
Product Source: E.coli

Aplicação

For most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.

Ligação

Replaces: GF017

forma física

Lyophilized from a 10mM Acetic Acid solution.

Armazenamento e estabilidade

The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in 10mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.

Nota de análise

Specific Activity: Human PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


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