CBL202
Anti-Vimentin Antibody, clone VIM 3B4
clone VIM 3B4, Chemicon®, from mouse
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
VIM 3B4, monoclonal
reatividade de espécies
chicken, amphibian, bovine, monkey, canine, human
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG2a
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
Informações sobre genes
human ... VIM(7431)
Especificidade
Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Imunogênio
Vimentin purified from bovine lens
Aplicação
Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Descrição-alvo
57 kDa
forma física
Format: Purified
Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Protein A purified
Armazenamento e estabilidade
Maintain at 2–8°C for up to 12 months from date of receipt.
Nota de análise
Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen.
Franke, W W and Moll, R
Differentiation, 36, 145-163 (1987)
Identification of differentially expressed genes in fetal rat forebrain exposed to a teratogen by cDNA microarray analysis.
S T Dheen,A J Hao,J Fu,P Gopalakrishnakone,E A Ling
Histology and Histopathology null
Cell type heterogeneity of intermediate filament expression in epithelia of the human pituitary gland.
Kasper, M, et al.
Histochemistry, 93, 93-103 (1989)
Lijie Liu et al.
Development, growth & differentiation, 49(8), 669-681 (2007-09-21)
To identify proteins involved in pancreatic development, we used a differential proteomics approach by comparing pancreatic extracts from four biologically significant stages of development: embryonic day (E) 15.5, E18.5, postnatal (P) days 0 and adult. By two-dimensional gel electrophoresis (2D-E)
Sites of origin and developmental dynamics of the neurons in the core and shell regions of torus semicircularis in the Chinese softshell turtle (Pelodiscus sinensis).
Chao Xi,Qiong Chen,Shao-Ju Zeng,Yu-Tao Lin,Yu-Fang Huang,Yu Liu,Xin-Wen Zhang,Ming-Xue Zuo
The Journal of Comparative Neurology null
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