ABE472

Anti-WAC Antibody, NT

from rabbit, purified by affinity chromatography

• Sinônimo(s): WW domain-containing adapter protein with coiled-coil
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, mouse
• técnica(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: NP_057712
• nº de adesão UniProt: Q9BTA9
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... WAC(51322)

Descrição

Descrição geral

WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at ′Lys-120′ (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the N-terminus of the WAC protein.

Imunogênio

Recombinant protein corresponding to human WAC.

Aplicação

Anti-WAC, N-terminus detects levels of WAC proteins & has been published & validated for use in WB, IP & ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 1 µg/mL from a representative lot detected WAC in 10 µg of HEK293 cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

Qualidade

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, N-terminus in 10 µg of K562 cell lysate.

Descrição-alvo

~80 kDa observed. Multiple isoforms are known to exist at ~70 kDa, ~65 kDa, ~60 kDa, and ~47 kDa Uncharacterized bands may be observed at ~52 kDa, ~75 kDa, and ~270 kDa in some cell lysates.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
K562 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable