ABD43

Anti-Apelin receptor Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Apelin Receptor, Angiotensin Receptor-Like 1, G-Protein Coupled Receptor Apj, G-Protein Coupled Receptor Hg11
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, mouse
• reatividade da espécie (prevista por homologia): rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: Np_005152
• nº de adesão UniProt: P35414
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... APLNR(187)

Descrição

Descrição geral

The Apelin receptor (APJ receptor) is a Gi/o-coupled protein with significant sequence homology to the angiotensin II type 1 (AT1) receptor. It is activated by apelin peptides (12, 17, or 36 amino acids). The effects of the apelin receptor are mediated mainly through the activation of protein kinase C and calcium signaling. The apelin receptor has been detected in the brain, heart, spleen, thymus, ovary, small intestine, and colon. Several studies have suggested that it is involved in regulating cardiovascular function and glucose homeostasis.

Especificidade

This antibody recognizes Apelin receptor at the cytoplasmic domain.

Imunogênio

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Apelin receptor.

Aplicação

Research Category
Stem Cell Research

This Anti-Apelin receptor Antibody is validated for use in Western Blotting, IHC(P) for the detection of Apelin receptor.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Apelin receptor on 10 µg of human cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in several regions of mouse brain sections.

Qualidade

Evaluated by Western Blot in mouse cerebellum tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Apelin receptor on 10 µg of mouse cerebellum tissue lysate.

Descrição-alvo

~62 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~62 kDa due to high glycosylation. (Medhurst, A., et al (2003). Journal of Neurochemistry. 84:1162–1172.)

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Mouse Cerebellum Tissue Lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable