AB6016

Anti-F-actin-capping protein subunit alpha-1 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): capping protein (actin filament) muscle Z-line, alpha 1, CapZ alpha-1, F-actin-capping protein subunit alpha-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: pig, mouse, human, rat
• reatividade da espécie (prevista por homologia): rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão UniProt: P52907
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: bovine ... Capza1(534656) ; dog ... Capza1(475857) ; human ... CAPZA1(829) ; mouse ... Capza1(12340) ; pig ... Capza1(100037957) ; rat ... Capza1(691149) ; rhesus monkey ... Capza1(705653)

Descrição

Descrição geral

F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm.

Imunogênio

GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1.

Aplicação

Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells.

Qualidade

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate.

Descrição-alvo

~35 kDa

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HeLa cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable