AB5156
Anti-Calcium Channel Antibody, Voltage Gated α 1C
Chemicon®, from rabbit
Sinônimo(s):
L-type
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
rat, rabbit, mouse, human
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CACNA1C(775)
Descrição geral
Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to dhp compounds (Swiss Prot).
Especificidade
Recognizes alpha 1C subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Guinea pig (17/18 residues identical); human and rabbit (16/18 residues identical).
Imunogênio
Purified peptide (C)TTKI NMDDL QPSEN EDKS (CNC1) from 848-865 of alpha 1C subunit of rat brain voltage-gated calcium channel (Accession number P22002). Epitope location is in the intracellular loop between domains II and III.
Aplicação
Alpha 1 subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Detect Calcium Channel using this Anti-Calcium Channel Antibody, Voltage Gated α 1C, validated for use in IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Ion Channels & Transporters
Signaling Neuroscience
Descrição-alvo
190 & 210 kDa
forma física
Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
ImmunoAffinity Purified
Armazenamento e estabilidade
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution add glycerol (ASC grade or better) at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Nota de análise
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Timothy syndrome is associated with activity-dependent dendritic retraction in rodent and human neurons.
Krey, JF; Pasca, SP; Shcheglovitov, A; Yazawa, M; Schwemberger, R; Rasmusson, R; Dolmetsch, RE
Nature Neuroscience null
State-dependent signaling by Cav1.2 regulates hair follicle stem cell function.
Yucel, G; Altindag, B; Gomez-Ospina, N; Rana, A; Panagiotakos, G; Lara, MF; Dolmetsch, R; Oro, AE
Genes & Development null
Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway.
Dolmetsch, R E, et al.
Science (New York, N.Y.), 294, 333-339 (2001)
Wei Wang et al.
Molecular medicine reports, 14(4), 3848-3854 (2016-09-08)
Remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (AF). Evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including Nkx2.5, are crucial for the development of AF. Rat atrial myocardial
Georgia Panagiotakos et al.
eLife, 8 (2019-12-24)
The syndromic autism spectrum disorder (ASD) Timothy syndrome (TS) is caused by a point mutation in the alternatively spliced exon 8A of the calcium channel Cav1.2. Using mouse brain and human induced pluripotent stem cells (iPSCs), we provide evidence that
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