AB4340
Anti-Zscan4 Antibody
from rabbit, purified by affinity chromatography
Sinônimo(s):
Zinc finger and SCAN domain containing protein 4C
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
mouse
técnica(s)
immunoprecipitation (IP): suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Descrição geral
Zinc finger and SCAN domain containing protein 4C (UniProt: Q80VJ6) is encoded by the Zscan4c (also known as Gm397) gene (Gene ID: 245109) in mouse. Zscan4 is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency, facilitate telomere elongation, and defy cellular senescence. It is expressed during zygotic genome activation in mouse two-cell embryos. It protects embryos from DNA damage by stabilizing the B-DNA form of microsatellite repeat DNA. It binds to the TGCACAC motif in CA/TG microsatellite repeats and regulates telomere elongation to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells. Dysfunction of Zscan4 during development or in mESCs leads to implantation failure, telomere maintenance defects, and chromosomal rearrangements. (Ref.: Srinivasan, R., et al., (2020), Sci. Adv., 6, eaaz9115)
Imunogênio
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of mouse Zscan4. The immunogen sequence is conserved between Zcsan4 family proteins (Zscan4b, Zscan4C, Zscan4d and Zscan4f).
Aplicação
Anti-Zscan4, Cat. No. AB4340, is a rabbit polyclonal antibody that detects Zscan4 and is tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualidade
Evaluated by Western Blotting in mouse embryonic stem cell (mESCs) lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Zscan4 in mouse embryonic stem cell (mESCs) lysate.
Descrição-alvo
Target molecular weight ~ 60 kDa observed; 60.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
forma física
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Recommended storage: +2°C to +8°C.
Nota de análise
Control
Embryonic Stem Cell lysates
Embryonic Stem Cell lysates
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Lei Yang et al.
EMBO reports, 21(9), e50054-e50054 (2020-07-28)
Cloned animals generated by somatic cell nuclear transfer (SCNT) have been reported for many years; however, SCNT is extremely inefficient, and zygotic genome activation (ZGA) is required for SCNT-mediated somatic cell reprogramming. To identify candidate factors that facilitate ZGA in
Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells.
Storm, MP; Kumpfmueller, B; Bone, HK; Buchholz, M; Sanchez Ripoll, Y; Chaudhuri, JB; Niwa et al.
Testing null
Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay.
Xiaoying Ye et al.
Frontiers in cell and developmental biology, 9, 675411-675411 (2021-06-15)
It has been well established that leukemia inhibitory factor (LIF) is essential for maintaining naïve pluripotency of embryonic stem cells (ESCs). Oncostatin M (OSM) is a member of the IL-6 family of cytokines which share gp130 as a receptor subunit
Role of Tet proteins in enhancer activity and telomere elongation.
Lu, F; Liu, Y; Jiang, L; Yamaguchi, S; Zhang, Y
Genes & Development null
Deepika Puri et al.
EMBO reports, 22(12), e53180-e53180 (2021-10-05)
Repeat element transcription plays a vital role in early embryonic development. The expression of repeats such as MERVL characterises mouse embryos at the 2-cell stage and defines a 2-cell-like cell (2CLC) population in a mouse embryonic stem cell culture. Repeat
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